2ZID

Crystal structure of dextran glucosidase E236Q complex with isomaltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	72.490, 82.530, 104.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.184
Rwork	0.184
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zic
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.000
High resolution limit [Å]	2.200
Number of reflections	32499
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM tris-HCl, 12% PEG6000, 200mM NaCl, 25mM isomaltotriose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K