2ZIC

Crystal structure of Streptococcus mutans dextran glucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-11-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	72.720, 86.470, 104.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 2.200
R-factor	0.183
Rwork	0.183
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uok
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.000
High resolution limit [Å]	2.200
Number of reflections	32892
Completeness [%]	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM tris-HCl, 12% PEG6000, 200mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K