2ZIB

Crystal structure analysis of calcium-independent type II antifreeze protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.275, 48.416, 59.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.340
R-factor	0.133
Rwork	0.131
R-free	0.16400
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.906
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.340
Number of reflections	28592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	0.1M MES, 2M AMMONIUM SULFATE, pH 5.40, VAPOR DIFFUSION, HANGING DROP, temperature 293K