2ZHZ
Crystal structure of a pduO-type ATP:cobalamin adenosyltransferase from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.23986 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.907, 148.174, 158.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.940 - 1.800 |
| R-factor | 0.211 |
| Rwork | 0.201 |
| R-free | 0.22900 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.940 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.249 |
| Number of reflections | 57707 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 97.8 | 88.8 |
| Redundancy | 6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | 0.1M Na Citrate, 22% Isopropanol, 12% PEG 4000, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
