2ZHO

Crystal structure of the regulatory subunit of aspartate kinase from Thermus thermophilus (ligand free form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2006-06-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 31
Unit cell lengths	107.179, 107.179, 87.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.410 - 2.980
R-factor	0.244
Rwork	0.244
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dt9
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.410	3.090
High resolution limit [Å]	2.980	2.980
Rmerge	0.092
Number of reflections	22878
<I/σ(I)>	21	3.23
Completeness [%]	99.8	100
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.6M sodium chloride, 0.1M Sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K