2ZHN

Crystal structure of human galectin-9 N-terminal CRD in complex with N-acetyllactosamine trimer (crystal 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-18
Detector	ADSC QUANTUM 210
Spacegroup name	P 41 21 2
Unit cell lengths	52.157, 52.157, 113.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.400 - 1.300
R-factor	0.182
Rwork	0.182
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.221
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.960	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	39617
Completeness [%]	100.0	100
Redundancy		9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1M TRI-SODIUM CITRATE (PH5.6), 1.0M MONO-AMMONIUM DEHYDRATE PHOSPHATE, pH 5.60, VAPOR DIFFUSION, SITTING DROP, temperature 293K