2ZHI
Crystal Structure Analysis of the Sodium-Bound Annexin A4 at 1.58 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.287, 40.244, 65.447 |
Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.580 |
R-factor | 0.1854 |
Rwork | 0.185 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ann |
RMSD bond length | 0.008 |
RMSD bond angle | 0.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.040 | 0.234 |
Number of reflections | 37926 | |
<I/σ(I)> | 18.8 | 6.3 |
Completeness [%] | 99.3 | 93.4 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 0.1M soduim citrate, 0.5M magnesium sulfate, 0.9M lithium sulfate, pH5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |