2ZHG
Crystal structure of SoxR in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 53.374, 53.374, 355.159 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 2.800 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.104 | 0.459 |
| Number of reflections | 7602 | |
| <I/σ(I)> | 13 | 2.6 |
| Completeness [%] | 91.1 | 58.4 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 | 50mM Bis-Tris pH 6.6-6.8, 0.3M K/Na tartrate, 15% (w/v) PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis-Tris | ||
| 2 | 1 | 1 | K/Na tartrate | ||
| 3 | 1 | 1 | PEG 10000 | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | Bis-Tris | ||
| 6 | 1 | 2 | K/Na tartrate | ||
| 7 | 1 | 2 | PEG 10000 | ||
| 8 | 1 | 2 | HOH |
