2ZH7
Complex structure of AFCCA with tRNAminiDG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.926, 57.926, 440.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.140 - 3.000 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 16195 | |
<I/σ(I)> | 34.1 | 4.5 |
Completeness [%] | 99.2 | 96.4 |
Redundancy | 11.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 50mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM NH4SO4, pH 7.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | HEPES | ||
10 | 1 | 2 | HOH | ||
2 | 1 | 1 | PEG 3550 | ||
3 | 1 | 1 | Tri-lithium citrate | ||
4 | 1 | 1 | NH4SO4 | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | HEPES | ||
7 | 1 | 2 | PEG 3550 | ||
8 | 1 | 2 | Tri-lithium citrate | ||
9 | 1 | 2 | NH4SO4 |