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2ZH5

Complex structure of AFCCA with tRNAminiDCU

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-5A
Synchrotron sitePhoton Factory
BeamlineBL-5A
Temperature [K]100
Detector technologyCCD
Collection date2006-12-18
DetectorADSC QUANTUM 315
Wavelength(s)1.0000
Spacegroup nameP 43 21 2
Unit cell lengths57.991, 57.991, 442.386
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution39.510 - 2.600
R-factor0.243
Rwork0.243
R-free0.29300
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.500
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.690
High resolution limit [Å]2.6002.600
Number of reflections24783
<I/σ(I)>41.25.5
Completeness [%]99.699.3
Redundancy11.57.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.529350mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM NH4SO4, pH 7.5, VAPOR DIFFUSION, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111HEPES
1012HOH
211PEG 3550
311Tri-lithium citrate
411NH4SO4
511HOH
612HEPES
712PEG 3550
812Tri-lithium citrate
912NH4SO4

219869

PDB entries from 2024-05-15

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