2ZGY
PARM with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 64.437, 64.437, 201.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.440 - 1.900 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 1MWM |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.326 |
| Number of reflections | 62671 | |
| <I/σ(I)> | 65.52 | 2.05 |
| Completeness [%] | 97.5 | 88.6 |
| Redundancy | 5.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 290 | 12% (W/W) PEG 8000, 0.4M SODIUM CHLORID, 0.4M MAGNESIUM ACETATE, 0.1M SODIUM CACODYLATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
