2ZGV
Crystal Structure of human phosphoglycerate kinase bound to D-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2007-05-22 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.956, 106.627, 50.350 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.000 |
| R-factor | 0.17782 |
| Rwork | 0.176 |
| R-free | 0.25828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.697 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.220 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | |
| Number of reflections | 16987 | |
| <I/σ(I)> | 11.3 | 2.9 |
| Completeness [%] | 0.7 | 0.31 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.6M NaKPO4, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
