2ZGU

Crystal structure Agrocybe aegerita lectin AAL mutant I144G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2006-12-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	42.170, 42.170, 135.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.800 - 2.400
R-factor	0.221
Rwork	0.221
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2ZGL
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.360	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.076	0.381
Number of reflections	10544
<I/σ(I)>	18	4.7
Completeness [%]	99.9	100
Redundancy	5.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2M sodium chloride, 25% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K