2ZGT

Crystal structure of Agrocybe aegerita lectin AAL mutant F93G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2006-12-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	42.750, 42.750, 127.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.960 - 2.800
R-factor	0.208
Rwork	0.208
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zgl
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.040	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.102	0.391
Number of reflections	6445
<I/σ(I)>	17.5	3.9
Completeness [%]	99.9	99.9
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K