2ZGN

crystal structure of recombinant Agrocybe aegerita lectin, rAAL, complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2007-05-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	53.450, 66.540, 58.080
Unit cell angles	90.00, 109.07, 90.00

Refinement procedure

Resolution	29.050 - 2.500
R-factor	0.223
Rwork	0.223
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zgl
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.050	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.086	0.303
Number of reflections	13435
<I/σ(I)>	14.8	3.7
Completeness [%]	99.8	99.9
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	50mM Sodium Acetate, 5% PEG1000, 5% PEG8000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K