2ZFY
Crystal structure of human Otubain 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.250, 50.886, 54.722 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 29.150 - 1.690 |
| R-factor | 0.19466 |
| Rwork | 0.192 |
| R-free | 0.23891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tff |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.435 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 1.690 |
| Number of reflections | 23651 |
| <I/σ(I)> | 18.7 |
| Completeness [%] | 97.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG 8000, 0.2 M Sodium acetate, 0.1 M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
