2ZFN
Self-acetylation mediated histone H3 lysine 56 acetylation by rtt109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.589, 68.612, 55.472 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 42.320 - 1.900 |
| R-factor | 0.18718 |
| Rwork | 0.185 |
| R-free | 0.22946 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2rim |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.460 |
| Number of reflections | 41056 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG, Tris, Ammonium citrate, glycerol, ethylene glycol, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
