2ZFM

Crystal Structure of the Kif1A Motor Domain After Mg Release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	CCD
Collection date	2005-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	41.767, 55.784, 155.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.440 - 2.310
R-factor	0.226
Rwork	0.226
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i5s
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	16786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K