2ZFJ

Crystal Structure of the Kif1A Motor Domain during Mg release: Mg-releasing Transition-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	93
Detector technology	CCD
Collection date	2005-03-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	40.142, 51.202, 157.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 3.200
R-factor	0.267
Rwork	0.267
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i5s
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	4812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K