2ZF8

Crystal structure of MotY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.17654
Spacegroup name	P 65 2 2
Unit cell lengths	104.081, 104.081, 133.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.000 - 2.850
R-factor	0.29
Rwork	0.290
R-free	0.31200
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	2.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.085	0.359
Number of reflections	10468
<I/σ(I)>		2.7
Completeness [%]	99.6	100
Redundancy	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	1.12M sodium potassium phosphate, 5% PEG1000, 0.1M acetate, pH4.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	1.12M sodium potassium phosphate, 5% PEG1000, 0.1M acetate, pH4.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K