2ZF5
Crystal Structure of highly thermostable glycerol kinase from a hyperthermophilic archaeon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-18 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | H 3 |
| Unit cell lengths | 217.477, 217.477, 66.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.100 - 2.400 |
| R-factor | 0.17803 |
| Rwork | 0.174 |
| R-free | 0.25493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gla |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.472 |
| Number of reflections | 45951 | |
| <I/σ(I)> | 17.2 | 1.4 |
| Completeness [%] | 98.2 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG400, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
