2ZE7

Crystal Structure of adenosine phosphate-isopentenyltransferase complexed with zinc ion and substrate analog, DMASPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	110
Detector technology	CCD
Collection date	2004-11-18
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 42 21 2
Unit cell lengths	97.933, 97.933, 65.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.170 - 2.100
R-factor	0.19508
Rwork	0.192
R-free	0.25443
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.027
RMSD bond angle	2.605
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	69.170
High resolution limit [Å]	2.100
Number of reflections	17544
Completeness [%]	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	1.4-2.0M sodium formate, 5mM magnesium chloride, 0.1M sodium acetate buffer. 30mM zinc chloride, 20mM dimethylallyl S-thiolodiphosphate, pH 4.8-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K