2ZE5

Crystal Structure of adenosine phosphate-isopentenyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	110
Detector technology	CCD
Collection date	2004-01-01
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9792
Spacegroup name	P 42 21 2
Unit cell lengths	96.392, 96.392, 65.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.200 - 2.310
R-factor	0.2035
Rwork	0.201
R-free	0.25433
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	2.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	68.200
High resolution limit [Å]	2.310
Number of reflections	13741
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	1.4-2.0M sodium formate, 5mM magnesium chloride, 0.1M sodium acetate buffer, pH 4.8-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K