2ZE3

Crystal Structure of DFA0005 Complexed with alpha-Ketoglutarate: A Novel Member of the ICL/PEPM Superfamily from Alkali-tolerant Deinococcus ficus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-25
Detector	ADSC QUANTUM 4
Spacegroup name	P 63 2 2
Unit cell lengths	108.840, 108.840, 114.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.850 - 1.650
R-factor	0.223
Rwork	0.223
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.026
RMSD bond angle	2.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Rmerge	0.059
Number of reflections	189632
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	VAPOR DIFFUSION, SITTING DROP, temperature 298K