2ZE0
Alpha-glucosidase GSJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-05 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.829, 90.621, 72.285 |
| Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.20462 |
| Rwork | 0.202 |
| R-free | 0.24681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uok |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.277 |
| Number of reflections | 39052 | |
| <I/σ(I)> | 29.3 | 6.3 |
| Completeness [%] | 93.0 | 81.5 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 20% PEG 4000, 0.1M Na citrate buffer, 4.5mM D-(+)-glucono 1,5-lactone, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
