2ZDJ
Crystal Structure of TTMA177, a Hypothetical Protein from Thermus thermophilus phage TMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.421, 63.716, 71.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.860 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.292 |
| Number of reflections | 15444 | |
| <I/σ(I)> | 31.44 | 8.125 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15% (w/v) PEG 8000, 75mM MES, 150mM Calcium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
