2ZDJ

Crystal Structure of TTMA177, a Hypothetical Protein from Thermus thermophilus phage TMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97893
Spacegroup name	P 21 21 21
Unit cell lengths	63.421, 63.716, 71.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.860 - 2.200
R-factor	0.21
Rwork	0.210
R-free	0.26300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.292
Number of reflections	15444
<I/σ(I)>	31.44	8.125
Completeness [%]	100.0	100
Redundancy	10.1	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	15% (w/v) PEG 8000, 75mM MES, 150mM Calcium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K