2ZD7

The structure of VPS75 (Vacuolar protein sorting-associated protein 75)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	298
Detector technology	CCD
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	80.692, 84.580, 86.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
Rwork	0.232
R-free	0.25500
Structure solution method	MAD
RMSD bond angle	0.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.850	1.850
Rmerge	0.081	0.421
Number of reflections	48480
<I/σ(I)>	11.4	4.1
Completeness [%]	95.3	93
Redundancy	8	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	PEG4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K