2ZCZ
Crystal structures and thermostability of mutant TRAP3 A7 (ENGINEERED TRAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 190 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 |
| Unit cell lengths | 110.137, 110.137, 36.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19618 |
| Rwork | 0.194 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.304 |
| Number of reflections | 39479 | |
| <I/σ(I)> | 10.7 | 9 |
| Completeness [%] | 94.7 | 70.3 |
| Redundancy | 4.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M sodium citrate pH5.5, 30%(w/v)MPD, 0.2M ammonium acetate, 10mM L-tryptophan, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
