2ZCU

Crystal structure of a new type of NADPH-dependent quinone oxidoreductase (QOR2) from escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-10
Detector	BRUKER PROTEUM 300
Wavelength(s)	0.97934, 0.97947, 0.97167, 1.12714
Spacegroup name	P 32 2 1
Unit cell lengths	81.685, 81.685, 76.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.740 - 1.800
R-factor	0.236
Rwork	0.236
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	SMART
Data scaling software	SAINT (& PROSCALE)
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	28204
Completeness [%]	97.9	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	285	PEG 4000, COPPER CHLRORIDE, AMMONIUM SULFATE, HEPES, pH 7.50, EVAPORATION, temperature 285K