2ZCN
Crystal structure of IcaR, a repressor of the TetR family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.110, 86.140, 113.850 |
| Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.172 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zcm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | 0.359 |
| Number of reflections | 57448 | |
| <I/σ(I)> | 17.6 | 3.5 |
| Completeness [%] | 97.7 | 89.1 |
| Redundancy | 6.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 4~6% (w/v) PEG 1500, 1.4~1.8 M NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
