2ZCJ
Ternary structure of the Glu119Gln M.HhaI, C5-Cytosine DNA methyltransferase, with unmodified DNA and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 98.470, 98.470, 321.615 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.640 - 2.750 |
| Rwork | 0.218 |
| R-free | 0.26600 |
| Structure solution method | simulation annealing |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.750 |
| High resolution limit [Å] | 2.500 | 2.600 |
| Rmerge | 0.106 | 0.365 |
| Number of reflections | 18897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 100 mM Sodium Citrate, 1.6-2.1 Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | sodium citrate | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | H2O |
