2ZCA
Crystal Structure of TTHB189, a CRISPR-associated protein, Cse2 family from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97897 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.081, 71.238, 53.753 |
| Unit cell angles | 90.00, 115.78, 90.00 |
Refinement procedure
| Resolution | 39.170 - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.278 |
| Number of reflections | 32767 | |
| <I/σ(I)> | 27.8 | 3.92 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 30% PEG 600, 5% PEG 1000, 0.1M Sodium Cacodylate, 10% Glycerol, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
