2ZC8
Crystal structure of N-Acylamino Acid Racemase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0702, 1.0713, 1.0716 |
| Spacegroup name | I 4 |
| Unit cell lengths | 136.709, 136.709, 86.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.840 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 58049 | |
| <I/σ(I)> | 11.9 | 6.6 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1M HEPES, 10%(w/v) PEG 6000, 12%(v/v) MPD, 10%(v/v) glycerol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
