2ZC2
Crystal structure of DnaD-like replication protein from Streptococcus mutans UA159, gi 24377835, residues 127-199
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.701, 81.701, 52.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.860 - 2.100 |
R-factor | 0.246 |
Rwork | 0.243 |
R-free | 0.28800 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.720 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.527 |
Number of reflections | 22797 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.2 | 93 |
Redundancy | 8.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 20% v/v 1,4-butanediol, 0.1M Imidazole, 0.2M Zinc acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |