2ZBQ

Crystal structure of ribosomal protein L11 methyltransferase from Thermus thermophilus in complex with S-adenosyl-L-homocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Detector technology	CCD
Collection date	2003-10-20
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	82.912, 75.685, 61.931
Unit cell angles	90.00, 130.95, 90.00

Refinement procedure

Resolution	31.310 - 2.400
R-factor	0.241
Rwork	0.241
R-free	0.29200
Structure solution method	RIGID-BODY REFINEMENT
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	11340
<I/σ(I)>	25.1	4.1
Completeness [%]	99.5	99.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	MES, ammonium sulfate, dioxane, 1,6-hexanediol, pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K