2ZBP

Crystal structure of ribosomal protein L11 methyltransferase from Thermus thermophilus in complex with S-adenosyl-L-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Detector technology	CCD
Collection date	2002-06-09
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	82.454, 75.751, 62.077
Unit cell angles	90.00, 130.56, 90.00

Refinement procedure

Resolution	48.280 - 2.300
R-factor	0.209
Rwork	0.209
R-free	0.24600
Structure solution method	RIGID-BODY REFINEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	12983
<I/σ(I)>	20.2	5.1
Completeness [%]	99.9	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	MES, ammonium sulfate, dioxane, 1,6-hexanediol, pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K