2ZBL
Functional annotation of Salmonella enterica yihS-encoded protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-24 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.229, 89.807, 94.730 |
| Unit cell angles | 106.86, 104.43, 110.31 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.15363 |
| Rwork | 0.152 |
| R-free | 0.18158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2afa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.376 |
| Number of reflections | 297314 | |
| <I/σ(I)> | 10.6 | 1.3 |
| Completeness [%] | 94.1 | 86.4 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15% PEG10000, 0.1M Tris-HCl, pH8.0, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
