2ZBJ
Crystal structure of Dioclea rostrata lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.511, 88.220, 87.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.110 - 2.050 |
| R-factor | 0.18936 |
| Rwork | 0.187 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h9w |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.986 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.150 | 2.103 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.067 | 0.341 |
| Number of reflections | 14526 | |
| <I/σ(I)> | 2.1 | 2.1 |
| Completeness [%] | 93.0 | 99.8 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Na-HEPES pH 7.5-8.5, 0.4-1.2M sodium potassium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
