2ZBI
Crystal structure of a bacterial cell-surface flagellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Detector technology | CCD |
| Collection date | 2006-09-25 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.486, 52.166, 76.198 |
| Unit cell angles | 99.11, 93.90, 107.97 |
Refinement procedure
| Resolution | 37.680 - 2.000 |
| R-factor | 0.2008 |
| Rwork | 0.196 |
| R-free | 0.28210 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.711 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.935 | |
| Number of reflections | 61347 | |
| <I/σ(I)> | 23.93 | 6.74 |
| Completeness [%] | 97.4 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 28% PEG 5000 MME, 0.2M Ammonium sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
