2ZB6

Crystal structure of the measles virus hemagglutinin (oligo-sugar type)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-13
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	134.092, 134.092, 100.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.225
Rwork	0.225
R-free	0.24800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.080	0.644
Number of reflections	28599
<I/σ(I)>	11.6	4.3
Completeness [%]	99.2	99.9
Redundancy	12.1	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2M sodium formate, 0.1M sodium acetate trihydrate, 10% ethylene glycol, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K