2ZB5
Crystal structure of the measles virus hemagglutinin (complex-sugar-type)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.523, 134.523, 99.251 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
Rwork | 0.227 |
R-free | 0.27110 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.462 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.064 | 0.388 |
Number of reflections | 18688 | |
<I/σ(I)> | 9.4 | 5.11 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 200mM NaCl, 100mM sodium cacodylate, pH 6.5, 10% polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |