2ZAV
Arginase I (homo sapiens): native and unliganded structure at 1.70 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 3 |
| Unit cell lengths | 90.480, 90.480, 69.449 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| Rwork | 0.143 |
| R-free | 0.17580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AEB: chain A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.416 |
| Number of reflections | 68183 | |
| <I/σ(I)> | 3.5 | 3.52 |
| Completeness [%] | 97.3 | 100 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 14% JEFFAMINE 2001, 0.1M HEPES pH 7.0, 50mM BICINE pH 8.5, 1.4mM Tymine, VAPOR DIFFUSION, HANGING DROP |
