2ZAO

Crystal structure of mouse SKD1/VPS4B ADP-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	75.673, 75.673, 132.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.200
Rwork	0.225
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xwi
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.092	0.690
Number of reflections	7181
<I/σ(I)>	11	2.8
Completeness [%]	99.9	99.4
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	5% PEG3350, 0.1mM MES, ADP soating , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K