2ZAB
Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Cmplex with Product (GGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.730, 68.102, 79.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.850 - 1.660 |
| R-factor | 0.16159 |
| Rwork | 0.160 |
| R-free | 0.19379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cws |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.043 | 0.197 |
| Number of reflections | 22667 | |
| <I/σ(I)> | 56.7 | 5.9 |
| Completeness [%] | 97.7 | 95.6 |
| Redundancy | 6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 10% PEG 1000, 15% PEG 8000, 0.2M calcium acetate, 0.05M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
