2ZA2
Crystal Structure of the apo-form of orotidine-5'-monophosphate decarboxylase from P.falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-08-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.977 |
| Spacegroup name | H 3 |
| Unit cell lengths | 201.800, 201.800, 44.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.300 - 2.700 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f84 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.277 |
| Number of reflections | 18313 | |
| <I/σ(I)> | 12.4 | 4 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 23% PEG 3000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
