2Z9X

Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-L-alanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-13
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	68.720, 68.720, 311.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.920 - 1.940
R-factor	0.14657
Rwork	0.145
R-free	0.18370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2Z9U
RMSD bond length	0.013
RMSD bond angle	1.342
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.071	0.270
Number of reflections	56030
Completeness [%]	98.6	97
Redundancy		9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.05	277	0.1M HEPES, 2M Ammonium sulfate, 5mM Pyridoxyl-L-alanine, pH 8.05, VAPOR DIFFUSION, SITTING DROP, temperature 277K