2Z9U

Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti at 2.0 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-04-10
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	68.545, 68.545, 311.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.15809
Rwork	0.156
R-free	0.20553
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1VJO
RMSD bond length	0.015
RMSD bond angle	1.440
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.084	0.379
Number of reflections	58804
<I/σ(I)>	10.6
Completeness [%]	97.8	97.2
Redundancy	5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.05	277	0.1M HEPES, 2M Ammonium sulfate, pH 8.05, VAPOR DIFFUSION, SITTING DROP, temperature 277K