2Z9O
Crystal structure of the dimeric form of RepE in complex with the repE operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.729, 99.316, 95.003 |
| Unit cell angles | 90.00, 108.55, 90.00 |
Refinement procedure
| Resolution | 45.030 - 3.140 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rep |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 16535 | |
| <I/σ(I)> | 11 | 3.9 |
| Completeness [%] | 88.3 | 73.6 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 10% PEG 4000, 200mM Sodium Chloride, 5mM Samarium Chloride, 100mM HEPES-NaOH, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Sodium Chloride | ||
| 3 | 1 | 1 | Samarium Chloride | ||
| 4 | 1 | 1 | HEPES-NaOH | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | Sodium Chloride | ||
| 7 | 1 | 2 | Samarium Chloride | ||
| 8 | 1 | 2 | HEPES-NaOH |
