2Z9H
Ethanolamine utilization protein, EutN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.281, 66.540, 72.004 |
| Unit cell angles | 90.00, 119.25, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.710 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hd3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.820 | 2.700 |
| Rmerge | 0.103 | 0.059 | 0.494 |
| Number of reflections | 15586 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 97.0 | 96.8 | 93.5 |
| Redundancy | 2.4 | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 0.1M Bis-Tris, 0.2M ammonium acetate, 50% MPD, pH5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
