2Z97
Crystal Structure of Ferric Cytochrome P450cam Reconstituted with 7-Methyl-7-depropionated Hemin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-24 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.493, 66.124, 104.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.630 - 1.800 |
| R-factor | 0.15595 |
| Rwork | 0.153 |
| R-free | 0.20277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.764 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (9.2.10) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.630 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.348 |
| Number of reflections | 41567 | |
| <I/σ(I)> | 5.7 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 14% PEG 8000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
